HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells
Fig 5
Stabilization and transcriptional activity of HIFs during in vitro differentiation.
The protein levels of (A) HIF-1α and (B) HIF-2α were evaluated in wild type and HIF-1α deficient cells by western blot and normalized to the vinculin signal. Data are presented as means ± SEM. The densitometric analysis is representative of 3 independent experiments; # p < 0.05, ## p < 0.01, ### p < 0.001 (compared to parental mESC). (C) The mRNA levels of HIF-1α target genes glucose transporter 1 (Glut1) and vascular endothelial growth factor (Vegf) were measured by real-time quantitative PCR analysis. Data are presented as mean ± SEM from at least 4 independent experiments; * p < 0.05, *** p < 0.001 (HIF-1α+/+ vs. HIF-1α-/- cells); ### p < 0.001 (compared to parental mESC).