A Farnesyltransferase Acts to Inhibit Ectopic Neurite Formation in C. elegans
Fig 2
The nde-4 gene encodes the farnesyltransferase (FTase) β subunit (fntb-1).
(A) nde-4 mutants were mapped to a small interval on chromosome V and rescued with a genomic fragment containing the fntb-1 (F23B12.6) gene and an FNTB-1::GFP-translational fusion driven from the fntb-1 promoter. Promoter and genomic regions used in rescuing constructs and a Pfntb-1::GFP transcriptional reporter are delineated. (B) Quantification of VC4 and VC5 ectopic neurite rescue in nde-4(zy7) mutants by fntb-1 genomic, FNTB-1::GFP and unc-4 promoter driven FNTB-1 constructs. Representative extrachromosomal lines are shown. Error bars represent the standard error of the proportion, n>200. *p<0.001, t test.