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Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells

Fig 3

A-B: Representative immunoblots and optical densities of total and phosphorylated TIE-2 (Tyr992), AKT (Ser473) and ERK1/2 (Thr202/Tyr204) after 10 min of exposure to Ang-1 (300 ng/ml) in HUVECs pre-incubated for 1 hr with PBS (control) or wild-type Fibulin-5 (90 μg/ml). Values are means ± SEM. N = 4 per group. *p<0.05 compared with control. #p<0.05 compared with Ang-1 alone. Note that Fibulin-5 attenuated Ang-1-induced TIE-2 phosphorylation, strongly inhibited both basal and Ang-1-induced AKT phosphorylation, and stimulated basal ERK1/2 phosphorylation. C: Representative immunoblots of total and phosphorylated AKT and ERK1/2 in HUVECs pre-incubated for 1 hr with PBS (0 time point) or increasing concentrations of recombinant wild-type Fibulin-5. Note that Fibulin-5 strongly inhibited AKT phosphorylation and augmented ERK1/2 phosphorylation.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0156994.g003