Campylobacter jejuni CsrA Regulates Metabolic and Virulence Associated Proteins and Is Necessary for Mouse Colonization
Fig 1
C. jejuni CsrA regulates FlaA expression by binding to the 5’ end of flaA mRNA.
(A) WT and csrA mutant C. jejuni were diluted to an initial OD600 = 0.1 and grown at 42°C. Cells were removed at 5, 6, 8 and 10 hours, and were used in western blots with antibodies against FlaA. (B) Electrophoretic Mobility Shift Assay (EMSA) analysis was used to assess the interaction between purified C. jejuni CsrA-His6 and the 5’ end of flaA mRNA. Purified CsrA-His6 at concentrations varying from 0–300 nM were incubated with 100 ng of purified 32P-end-labeled RNA corresponding to the 5’ ends of either C. jejuni flaA (top panel) or E. coli phoB (bottom panel) as a control that does not bind CsrA [15]. The positions of bound (B) and free (F) RNA are shown.