Papaverine Prevents Vasospasm by Regulation of Myosin Light Chain Phosphorylation and Actin Polymerization in Human Saphenous Vein
Fig 7
Preincubation with papaverine leads to decreases in phosphorylation of the mypt1 in HSV.
HSV rings were suspended in a muscle bath and equilibrated in Kreb’s bicarbonate buffer for 2 h. Rings were untreated (Basal), treated with 1 μM norepinephrine (NE) for 5 minutes, 1 mM papaverine (Pap) for 10 min followed by 1 μM norepinephrine for 5 min or 1 mM papaverine (Pap) for 10 min. Tissues were snap frozen and protein lysates were separated on 4–20% criterion gels and transferred to nitrocellulose membrane. Mypt1 and phospho mypt1 were identified by western blotting using antibodies against mypt1(Santa Cruz, 1:500) and phospho mypt1(thr 696) antibodies (cell signal, 1:500), respectively, and the ratio of p-mypt1/total mypt1 was calculated. Representative western blot for mypt1 are shown in Panel A with the cumulative bar graph shown in Panel B. * Significant, N = 4, p<0.05.