Short-Fragment DNA Residue from Vaccine Purification Processes Promotes Immune Response to the New Inactivated EV71 Vaccine by Upregulating TLR9 mRNA
Fig 6
Secretion of cytokines in culture supernatants of spleen lymphocytes after stimulation with EV71 inactivated liquid bulk.
Spleen lymphocytes from C57BL/6 mice (n = 5 per group) were co-incubated with EV71 inactivated liquid bulk (100U/ml) for 48h. Untreated samples were used to determine the background of secretion of cytokines. Suspension was then centrifuged, and cytokines in the cell-free supernatants were tested by luminex method. Five cytokines (IL-1β, IL2, IL6, IL-10 and IFN-γ) were found to be significantly up-regulated after incubation.