The N-Terminus of the Floral Arabidopsis TGA Transcription Factor PERIANTHIA Mediates Redox-Sensitive DNA-Binding
Fig 2
Redox-dependent binding of selected Arabidopsis TGA TFs to the AAGAAT motif.
(A) Cladogram of Arabidopsis TGA TFs indicating clade memberships. (B) Schematic representation of selected TGA TF family members and respective cysteine residue positions. Orange boxes mark the bZIP domain and blue boxes two glutamine-rich regions. Cys340, Cys260 and Cys301 (red) are localized in a conserved position in the first glutamine-rich region. (C) Gel retardation analysis of selected TGA proteins incubated with the AAGAAT probe under reducing (0.9 mM DTT; red) or oxidizing (2 mM diamide; ox) conditions. Asterisk marks unbound AAGAAT probe.