Determinants of Cofactor Specificity for the Glucose-6-Phosphate Dehydrogenase from Escherichia coli: Simulation, Kinetics and Evolutionary Studies
Fig 4
G6PDH activity of wild type and mutant enzymes as a function of NAD+ or NADP+ concentration.
NADP+-dependent activity (black circles) and activity with NAD+ (white circles) was measured at nearly saturating co-substrate concentrations (bottom right corner). The data was fitted to the Michaelis-Menten equation and the Pearson correlation coefficient is indicated. The values for the kinetic parameters are given in Table 1.