AtNHX5 and AtNHX6 Are Required for the Subcellular Localization of the SNARE Complex That Mediates the Trafficking of Seed Storage Proteins in Arabidopsis
Fig 9
AtNHX5 and AtNHX6 are co-localized with SYP22 and VAMP727.
(A) Subcellular localization of RFP:NHX5 or RFP:NHX6 with SYP22:GFP. The RFP:AtNHX5 or RFP:AtNHX6 plasmids were transiently co-expressed in Arabidopsis leaf protoplasts with SYP22:GFP, respectively. Fluorescence was visualized by a confocal laser scanning microscope. (B) Subcellular localization of YFP:NHX5 or YFP:NHX6 with VAMP727:mCherry. The YFP:AtNHX5 or YFP:AtNHX6 plasmids were transiently co-expressed in Arabidopsis leaf protoplasts with VAMP727:mCherry, respectively. Fluorescence was visualized by a confocal laser scanning microscope. Bar = 10 μm in (A) and (B).