Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay
Fig 1
Schematic representation of ESAT-6 detection by the biotin-streptavidin interaction.
Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT-6 and biotinylated anti-mouse-IgG. Streptavidin-HRP with and without free biotin then was added.