Comprehensive Identification of Krüppel-Like Factor Family Members Contributing to the Self-Renewal of Mouse Embryonic Stem Cells and Cellular Reprogramming
Fig 7
Delineation of the domains of Klf2 required for the self-renewal of mouse ES cells.
(A) Schematic illustration of Klf2 deletion (Δ) mutants. Klf2 is highly homologous to Klf4 and belongs to the same subclass. A FLAG-HA tandem epitope tag was fused to the N-terminus of each of the deletion mutants. The zinc finger domain is shown as green boxes. (B) Western blot analyses of Klf2 and Klf2 deletion mutants with an anti-HA antibody. Arrowheads indicate HA-Klf proteins. (C) Percentage of AP-positive colonies in ES cells overexpressing full-length Klf2 or Klf2 deletion mutants, grown in the presence or absence of LIF. (D) Expression of the pluripotency-related marker Rex1. Rex1-GFP expression is seen in full-length Klf2, and N-terminal deletion mutants (N1, N2) in the absence of LIF. Scale bar: 100 μm. Asterisks and hash symbols indicate statistical significance. *P < 0.05 vs. empty vector; # P < 0.05 vs. full-length Klf; * P < 0.01; n.s.; not significant; n.d.; not detected.