Genome-Wide Investigation Using sRNA-Seq, Degradome-Seq and Transcriptome-Seq Reveals Regulatory Networks of microRNAs and Their Target Genes in Soybean during Soybean mosaic virus Infection
Fig 3
Stem-loop RT-qPCR analysis of miRNA expression in response to infection by three SMV isolates.
Small RNAs fractions (≤ 200 nt) derived from SMV-infected and mock-inoculated plants were isolated at 14 dpi. Soybean 18S rRNA was used as an internal control. Error bars represent mean ± SD (standard deviation) and the data are averages from three biological replicates. Asterisks indicate statistically significant differences comparing with the mock control (student’s t-tests) (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant).