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The Arabidopsis Domain of Unknown Function 1218 (DUF1218) Containing Proteins, MODIFYING WALL LIGNIN-1 and 2 (At1g31720/MWL-1 and At4g19370/MWL-2) Function Redundantly to Alter Secondary Cell Wall Lignin Content

Fig 2

Characterization of mwl-1 and mwl-2 insertion alleles and overall plant growth of the mwl-1/mwl-2 double knockout mutant.

(A) Genomic structure of MWL-1 and MWL-2 comprises three exons and two introns showing disruption by the T-DNA in exon 3 and 2, respectively. (B) RT-PCR detected endogenous MWL-1 and MWL-2 transcript in the wild-type plants (WT), while endogenous transcript was absent in the homozygous double knockout mutant. Actin2 was used as an internal control gene. Position of the oligonucleotides used for the RT-PCR is indicated in A by arrows and the sequences can be found in S1 Table. (C and D). Qualitative comparison of rosette (C) and leaves (D) of double mutant relative to the WT control plants after four weeks growth. (E) Qualitative comparison of single and double knockout inflorescence stems relative to WT control. (F and G) Quantitative analysis of four-week-old rosette leaves (n = 8, F), and six-week-old inflorescence stem length (n = 65) relative to WT control (G). Scale bar, 3 cm. Error bars are standard error and significant difference from the WT based on a paired two-tailed Student’s t-test (p-value ≤ 0.05) is indicated by an asterisk (*).

Fig 2

doi: https://doi.org/10.1371/journal.pone.0150254.g002