Contributions of EspA Filaments and Curli Fimbriae in Cellular Adherence and Biofilm Formation of Enterohemorrhagic Escherichia coli O157:H7
Fig 6
Effect of temperature and glucose on EspA and CsgA production and biofilm formation.
Bar graphs of biofilms produced in the wells of a microtiter plate after 48 h of growth at 37°C in DMEM (A) and YESCA-glucose broth (B). Bars represent means of three independent assays and error bars represent standard deviation of 2. The * above the bars indicates p < 0.05 when biofilm formation in the mutant strains was compared to the parental or the hha mutant strain. Western blot analysis of cell lysates prepared from bacterial strains grown in DMEM (C) and YESCA-glucose (D) and probed using anti-DnaK (top blot), anti-EspA (middle blot), and anti-CsgA (bottom blot) antibodies. Molecular weights of protein standards are indicated in kD on the right side of each blot. The locations of predicted or identified DnaK, EspA, and CsgA proteins are indicated on the left. Lanes numbered 1 through 9 contain cell lysates of strains listed in the same order as in Fig 6A and 6B.