RacA-Mediated ROS Signaling Is Required for Polarized Cell Differentiation in Conidiogenesis of Aspergillus fumigatus
Fig 2
The A. fumigatus ΔracA is defective in phialide development.
Agar blocks including aerial or embedded conidiophores were prepared from an A. fumigatus colony grown on GMM agar plate (30°C, 4 days after inoculation). Vesicles of the ΔracA are indicated with black arrowheads. (A and B) Aerial conidiophores of the wild type (A) and the ΔracA mutant (B). Bars = 50 μm. (C and D) Conidiophores of the wild type (C) and the ΔracA mutant (D) stained with 25 μM Calcofluor White. Bars = 15 μm. (E and F) Agar-embedded conidiophores of the wild type (E) and the ΔracA mutant (F). Bars = 50 μm. (G—I) Embedded conidiopohres of the wild type (G) and the ΔracA mutant (H and I) stained with 0.5 mM NBT.