Mechanism of Copper Uptake from Blood Plasma Ceruloplasmin by Mammalian Cells
Fig 8
Inhibition of uptake of 64Cu from Cp by an excess of non-radioactive ionic Cu(II), Cu(I), or Fe(III).
Uptake of 64Cu from purified mouse Cp over 3 h by MEF cells expressing and not expressing Ctr1 was measured in the absence and presence of non-radioactive 150 μM Cu(II) and Cu(I) (A) or Fe(III) (B) as the 5:1 NTA:metal complex. Results for cells with and without Ctr1 were similar and so were pooled, and are given as Means ± SD, N = 12 for Cu(II), 6 for Cu(I), and 6 for Fe(III). *p <0.001 for difference from controls.