Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Microparticle-Induced Activation of the Vascular Endothelium Requires Caveolin-1/Caveolae

Fig 9

Caveolae endocytosis inhibition attenuates MP-induced phosphorylation of p65 and EGFR.

MLECs were pretreated with 60 μM Dynasore or 0.1% DMSO for 30 min prior to incubation with MPs for indicated time points (0, 0.5, 1, 2 hrs). Cells were then harvested, separated by SDS-Page and blotted for the proteins indicated. Inhibition of caveolae endocytosis attenuated MP-induced phosphorylation of p65 (A) and EGFR (B) at 30 min. (Avg ± SEM two-way ANOVA and bonferroni post hoc analysis for p65 and Kruskal-Wallis test **p<0.01 for EGFR A. p65: n = 6 dmso n = 4 dynasore # denotes comparison with DMSO t = 0, ###p<0.001 B. n = 4 for each condition *p<0.05).

Fig 9

doi: https://doi.org/10.1371/journal.pone.0149272.g009