Voltage-Gated K+ Channel, Kv3.3 Is Involved in Hemin-Induced K562 Differentiation
Fig 5
Signaling mechanisms of K562 erythroid differentiation regulation by siRNA- Kv3.3 transfection.
The expressions of p38, ERK1/2, CREB, and c-fos during cell differentiation changed with siRNA-Kv3.3 transfection. The levels of the phosphorylated, activated forms of p38 and CREB were lower in siRNA-Kv3.3 transfected cells than in control cells. The levels of c-fos during cell differentiation were also reduced after siRNA- Kv3.3 transfection. The levels of phosphorylated ERK2 (p-42 MAPK) also seemed lower; however, the differences were not statistically significant. Phosphorylated ERK1 (p-44 MAPK) was not detected. No changes were noted for the total p38, total ERK1/2, and total CREB. The graphs show the quantitative analysis of each protein. Western blot assay was performed when transfected cells were differentiated for 48 h with hemin; each assay was performed in triplicate, and data are expressed as mean ± standard error. **p<0.01 compared with control value. The relative protein expressions of the signal molecules were expressed as a fold change relative to the control group.