Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

A Development of Nucleic Chromatin Measurements as a New Prognostic Marker for Severe Chronic Heart Failure

Fig 3

Protocol to Quantitatively Measure Chromatin Area in Group N.

Our novel method is composed of the following steps. (A) Representative image prior to the availability of digital analysis (10,000× magnification). (B) The perinuclear condensed chromatin was isolated automatically by image analysis software. The area of the intranuclear chromatin excluding the nucleolus, shown as (b), was measured. (C) The area of condensed chromatin, defined as the area with a grayscale value between 193 and 256, was measured (red). The nucleoplasmic chromatin score (Nuc-CS) was defined as (c) / (b) ×100 (%). (D) The area of perinuclear condensed chromatin, defined as (d), and the length of the inner nuclear perimeter were automatically measured (outlined in red in (D)). The perinuclear chromatin score (Per-CS) was defined as (d) / the length of the inner nuclear perimeter (pixel).

Fig 3

doi: https://doi.org/10.1371/journal.pone.0148209.g003