Studying Coxiella burnetii Type IV Substrates in the Yeast Saccharomyces cerevisiae: Focus on Subcellular Localization and Protein Aggregation
Fig 6
Localization of GFP-AnkA to the yeast IPOD compartment.
(A) YPH499 transformants expressing GFP-AnkA (green) from the pYES2-GFP-AnkA plasmid after induction in galactose for 4 h were incubated with FM4-64 (red) for 30 min to allow visualization of the vacuolar membrane. (B) YPH499 yeast cells were co-transformed with pYES2-GFP-AnkA and p425-GAL1-RNQ1-mCherry, an induced as above. Representative cells are shown of two typical patterns: neat co-localization of both red and green channels (upper panel) and inclusion of the green GFP-AnkA signal within a larger Rnq1-mCherry compartment (lower panel). Bars represent 5 μm.