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Distribution of T Cells in Rainbow Trout (Oncorhynchus mykiss) Skin and Responsiveness to Viral Infection

Fig 4

Distribution of trout CD3+ cells in anterior and posterior skin regions.

Inmunohistochemical detection of trout CD3+ cells in different skin areas of anterior (A) and posterior (B) body sections. Counterstained with Mayer’s haematoxylin. Scale bar represents 100μm. Total numbers of CD3+ cells for each skin section were calculated using the imaging analysis Image J software (NIH). Then, relative number of cells per mm2 were calculated and plotted. Data are shown as mean ± SD (n = 10). Asterisks indicate mean values in posterior sections significantly lower than values in anterior areas (P < 0.001).

Fig 4

doi: https://doi.org/10.1371/journal.pone.0147477.g004