The TLR4 Agonist Vaccine Adjuvant, GLA-SE, Requires Canonical and Atypical Mechanisms of Action for TH1 Induction
Fig 3
IFNαR1 signaling is essential for lymphocyte activation and IFNγ production upon immunization with ID93+GLA-SE.
B6 mice were treated with IFNαR1 antibody or its isotype and immunized with ID93+GLA-SE. Innate responses were assessed in the ipsilateral draining LN or in the contralateral LN by flow cytometry on LN cells or ELISA on LN supernatant The results are shown for the ipsilateral LN when not stated otherwise. (A) MFI and representative histograms of CD69 staining (B) IFNγ production assessed by ELISA, (C) IFNγR occupancy as indicated by decreased MFI of IFNγR staining with the monoclonal antibody GR-20 at 12 hrs after immunization. (D) IFNγ staining on CD8+ T cells. (E) IFNγ staining on NK cells. (F) IL-12 production assessed by ELISA. Data are shown as mean ± SEM of N = 4 animal/group and are from one experiment. Statistics by one-way ANOVA, *p≤0.05 compare to anti-IFNαR1 treated animals, #p≤0.05 compare to all the other groups.