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Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells

Fig 2

Top: western blotting of the levels of pro-caspase-9 and caspase-9; centre: pro-caspase-8 and caspase-8; and bottom: pro-caspase-3 and caspase-3 proteins.

Caco-2 cells were treated with MA at IC50 and IC80 concentrations for 4 h. The levels of protein expression are expressed as arbitrary intensity units of each band compared to arbitrary intensity units of actin. The values represent means ± S.D. of at least three independent experiments performed in triplicate. Key: (*) p<0.05, (**) p<0.01 and (***) p<0.001, with respect to the untreated control cells.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0146178.g002