Selecting Superior De Novo Transcriptome Assemblies: Lessons Learned by Leveraging the Best Plant Genome
Fig 5
Normalization improves the recovery rate of highly expressed genes (compare to Fig 4).
The units for “Assembly Quality” are Normalized Bit Score (BS) and the units for “Sequence Depth” are Sequenced Fragments/bp (SFB). The number printed in the plot area is the number of unigenes with normalized bit score above 1.5. A BS of 1.5 is an arbitrary threshold and is used to illustrate the differences in the high-density region seen in most plots near BS 1.75–2.