Expression Profiles of Long Noncoding RNAs and Messenger RNAs in Mn-Exposed Hippocampal Neurons of Sprague–Dawley Rats Ascertained by Microarray: Implications for Mn-Induced Neurotoxicity
Fig 3
Overview of the microarray signatures.
(A) Scatter plots showing the variations in the mRNA expression between two groups (1, L/C; 2, M/C; 3, H/C). A scatter plot is a visualization method used to assess the variation (or reproducibility) between chips. The values of the X and Y axes are the averaged normalized signal values of groups of samples (X: log2 scaled; and Y: −log10 scaled). The green lines are fold change lines (FC = 2). The color of the points indicates the intensities from low (blue) to high (red). The mRNAs above the top green line and below the bottom green line indicate 2 FC between two groups of samples. (B) Volcano plots of the differentially expressed mRNAs (1, L/C; 2, M/C; 3, H/C). A volcano plot is a useful tool for visualizing differential expression between two different conditions. The vertical lines correspond to twofold up and down, and the horizontal line represents a p-value of 0.05. The red point in the plot represents the differentially expressed mRNAs with statistical significance.