A Multi Targeting Conditionally Replicating Adenovirus Displays Enhanced Oncolysis while Maintaining Expression of Immunotherapeutic Agents
Fig 1
(A) The two step HR strategy employed to generate the Ad5/3-C-RGD D24 genome. (B) PCR of the fiber region using adenoviral DNA prepared from purified virus particles. Top panel represents PCR products amplified using specific primer sets for Ad3 knob region. Bottom panel represents PCR products amplified using specific primer sets for Ad3 knob region and RGD on Ad3 knob C-terminus. (C) Schematic representation of the single and multi-targeting viruses.