Discovering Molecules That Regulate Efferocytosis Using Primary Human Macrophages and High Content Imaging
Fig 4
Phagocytic Index (PI) provides a direct measure of macrophage function.
Comparison of data presented as either PI (A) or representing the frequency of macrophages containing apoptotic cells expressed as “percent (%) positive cells” (B) within the same population in each well of a 96 well plate. The PI was calculated as described in Fig 3. Calculation of the % positive cells is based on the assumption that macrophages (APC+) containing apoptotic cells (Cy3+) appear fluorescent for both fluorophores and calculated as follows: % positive cells = (# APC+ Cy3+ cells / # APC+ cells) * 100. Ratios listed along the X-axis represent the (Target) T: MΦ ratios. Baseline engulfment was determined using MΦ’s co-cultured with live cells (Live); Negative controls consisted of cytochalasin D treated MΦ’s co-cultured with apoptotic cells (Neg). Test samples consisted of apoptotic cells co-cultured with MΦ’s (Apop).