Luteolin Inhibits Ischemia/Reperfusion-Induced Myocardial Injury in Rats via Downregulation of microRNA-208b-3p
Fig 4
AV/PI detected the early apoptotic cell percentage of H9c2 cells.
A. AR significantly increased the early apoptotic cell percentage of H9c2 cells compared with control group (**p<0.01). However, LUT pretreatment significantly decreased the apoptosis rate compared to AR group (#p<0.05). B. Overexpression of miR-208b-3p under AR significantly increased the early apoptotic cell percentage of H9c2 cells compared with AR group (***p<0.001). However, LUT pretreatment had no statistical significance compared to 208m+AR group (p>0.05). C. Knocking down miR-208b-3p expression under AR decreased the early apoptotic cell percentage of H9c2 cells compared with Ni+AR group (*p<0.05). D. Knocking down Ets1 expression under AR increased the early apoptotic cell percentage of H9c2 cells compared with si-Nc+AR group (*p<0.05).