Suggested Involvement of PP1/PP2A Activity and De Novo Gene Expression in Anhydrobiotic Survival in a Tardigrade, Hypsibius dujardini, by Chemical Genetic Approach
Fig 2
Effects of inhibition of transcription and translation on anhydrobiotic survival.
(A) Scheme of chemical treatment and subsequent desiccation tolerance assay. Tardigrades were incubated in a chemical solution or 1% DMSO solution (control) for 5 h. After chemical treatment and 1 day preconditioning at 95% RH, one group was exposed to low relative humidity (10% RH) for 2 days (Low humidity exposure). The other group was exposed to high relative humidity (95% RH) for same period (High humidity exposure). (B, C) Effects of a transcription inhibitor (10 μM α-amanitin) on recovery rates at 1 h (B) and 24 h (C) after rehydration. Mean ± SD (N = 4; 20 tardigrades each). (D, E) Effects of translation inhibitor (250 μM cycloheximide) on recovery rates at 1 h (D) and 24 h (E) after rehydration. Mean ± SD (N = 3; 10 tardigrades each). Statistically significant differences among samples were determined by the Tukey-Kramer test (*, P<0.05; **, P<0.01). Low humidity, low humidity exposure; High humidity, high humidity exposure.