Inhibition of BACE1 Activity by a DNA Aptamer in an Alzheimer’s Disease Cell Model
Fig 1
Aptamer generation using the SELEX process.
(A) Seven M urea 8% denatured polyacrylamide gel electrophoresis was used to confirm ssDNA, which was separated by asymmetric PCR after each round. Lane 1: pUC18 DNA/Msp l marker. Lane 2: initial library Gp30. Lane 3: unequal length PCR products. Lane 4: target ssDNA. (B) Selected pool enrichment was monitored via indirect ELISA. (C-D) Secondary structures of aptamers A1 (C) and A4 (D) predicted by RNAstructure 5.7 software (Mathews lab, http://rna.urmc.rochester.edu/RNAstructure.html).