Hemorrhage Exacerbates Radiation Effects on Survival, Leukocytopenia, Thrombopenia, Erythropenia, Bone Marrow Cell Depletion and Hematopoiesis, and Inflammation-Associated microRNAs Expression in Kidney
Fig 1
CI reduces survival and water consumption.
Mice received 7.75–9.25 Gy radiation and/or 20% hemorrhage to establish following four treatment groups: Sham, Hemo RI, or RI+Hemo. All values are means ± SEM. (A) No mice died in Sham and Hemo groups (data not shown). Mice received 8.75 Gy, N = 16 per group. *p<0.05 vs. Sham or Hemo group; **p<0.05 vs. RI, determined by Mantel-Cox test. (B) Mice received 7.75 Gy, 8.25 Gy, 8.75 Gy, or 9.25 Gy, N = 16 per group. Their survival was monitored for 30 days. *p<0.05 vs. respective RI group, determined by Mantel-Cox test. (C) Mortality in % in function of radiation doses was graphed and the 50% lethal radiation dose over 30 days (LD50/30) was calculated. Their LD50/30s are 8.7 Gy for RI and 9.1 Gy for RI+Hemo, respectively, and DMF is 1.046. (D) Mice received 9.25 Gy, N = 16 per group. Their daily water consumption for first 7 days after RI was measured. The average volume of daily water consumption is 4.081 ± 0.084 mL/mouse (N = 16 over a 7-day period of time). *p<0.05 vs. respective RI time point, determined by Student’s t-test. RI: radiation injury; Hemo: 20% bleeding; Sham: free of any injury.