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A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box

Fig 4

Development of full-term offspring derived from embryos transported by parcel-delivery service.

(A–C) morulae/blastocysts from BDF1, C57BL/6 and ICR mouse strains, respectively, cultured in a warm box using microtubes with a gas-permeable film and placed in a plastic bag containing the special gas mixture, and then transported by parcel-delivery service. BDF1 mouse embryos were cultured for 96 h in the warm box, but the other embryos were cultured for 72 h. (D–F) Full-term offspring from BDF1, C57BL/6 and ICR mouse strains, respectively, were obtained through Cesarean section and fostered by another ICR female mouse. BDF1 offspring (colored pups in D) are shown at 1 week after birth. The other pups are shown just after Cesarean section.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0138854.g004