PKC and AKT Modulate cGMP/PKG Signaling Pathway on Platelet Aggregation in Experimental Sepsis
Fig 5
Effects of PI3K and AKT inhibitors on cGMP levels and AKT phosphorylation of LPS-injected rats.
Washed platelets (1.2 x 108 platelets/ml) were incubated with LY29004 (10 μM), API-1 (20 μM) or its vehicle (1% DMSO) for 3 min before addition of ADP (10 μM). (Panel A) Intraplatelet cGMP concentration (pmol/ml) in saline and LPS-treated rats. (Panels B and C) Samples (50 μg of protein/well) were loaded on SDS-PAGE 10% and analyzed by Western blot using anti-AKT or anti-phospho(Thr 308)-AKT antibodies. Graph shows densitometric analysis of immunoreactive bands. Results are shown as mean ± SEM values (n = 4–6 different animals). *P<0.05 compared with respective groups of saline-injected rats. **P<0.05 compared with ADP-stimulated platelets of LPS group. #P<0.05 compared with unstimulated platelets of LPS group.