Rapid Molecular Detection of Multidrug-Resistant Tuberculosis by PCR-Nucleic Acid Lateral Flow Immunoassay
Fig 6
RpoB primer designs for codon 526 (mutations Y and R) have been demonstrated above as examples. SM (single mutation) primers are designed with a single base mismatch at the 3’-terminus to complement MT templates. DM (double mutation) primers, which have also been designed to complement MT templates, carry an added mismatch at the third position from the 3’-terminus. The red colored bases indicate an intentional mismatch and the blue colored bases indicate a complementary base to the SM and DM primers.