Human Mesenchymal Stem Cells Retain Multilineage Differentiation Capacity Including Neural Marker Expression after Extended In Vitro Expansion
Fig 7
Neural transcription factors and cell surface CD marker expression.
Undifferentiated hMSCs express transcription factors and cell surface CD markers including stemness (CD73, CD271, HES1, KLF4, SOX5/6/9) and neuronal (CD24, CD56, CD200, CD304, MEF2C, PAX3/9) markers reported to influence neural differentiation. With the exception of CD73 and CD304, expressed at levels equal to CD44 (a cell surface marker of MSCs), these neural markers were expressed at levels 10-10000X lower than CD44.