High-Throughput siRNA Screening to Reveal GATA-2 Upstream Transcriptional Mechanisms in Hematopoietic Cells
Fig 1
GATA-2 1S expression in YN-1 cells.
Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting were performed to measure GATA-2 expression in six hematopoietic cell lines. (A) Quantitative RT-PCR for GATA-2 mRNA (mean ± standard error [SE], n = 3). GAPDH mRNA was used as a control. The GATA-2 mRNA expression level in YN-1 cells was set to 1. (B) Anti-GATA-2 western blotting analysis of whole-cell extracts from six cell lines. Alpha-tubulin was used as a loading control. The asterisk demotes cross-reactive band. (C) Quantitative RT-PCR for GATA-2 1S or 1G mRNA (mean ± SE, n = 3). GAPDH mRNA was used as a control. The GATA-2 1S and 1G mRNA expression levels in YN-1 cells were set to 1.