Targeting the Sonic Hedgehog-Gli1 Pathway as a Potential New Therapeutic Strategy for Myelodysplastic Syndromes
Fig 5
Gli1 silencing significantly sensitizes 5-aza-dC to inhibit MUTZ-1 cells.
Gli1 silenced and scramble MUTZ-1 cells were cultured with or without 5-aza-dC (2 μM) for 48 h. (A). CCK8 assays were performed to determine the proliferation of MUTZ-1 cells transfected with sh-Gli1 (Gli1 sh1 and Gli1 sh2) and control lentiviral vector (Scramble) in the presence of different concentrations of 5-aza-dC. Two-way ANOVA with post hoc analysis (Bonferroni test) was performed to determine the effects of Gli1 knockdown and 5-aza-dC on cell viability. (B). Representative images of cell cycle distribution (left panel), and the percentage of cells in the G0/G1, S, or G2/M phases of the cell cycle are indicated in the bar charts (right panel). (C). Representative images of cellular apoptosis determined by flow cytometry analysis (left panel), and the sum percentage of early and late apoptotic cells indicated in the bar charts (right panel). *P < 0.05. Results are expressed as mean ± SEM.