Next Generation Sequencing Analysis Reveals Segmental Patterns of microRNA Expression in Mouse Epididymal Epithelial Cells
Fig 8
qRT-PCR validation of differentially expressed miRNAs within the mouse epididymis.
In order to verify the next generation sequence data, nine differentially expressed miRNAs were selected for targeted validation using qRT-PCR, including representatives with highest expression in the proximal (caput: let-7c-5p, let-7b-5p, miR-375-3p, miR-9-5p, miR-467d-3p, and miR-200c-3p) and distal (cauda: miR-410-3p, miR-486-5p, and miR470c-5p) epididymis. qRT-PCR experiments were performed in triplicate using pooled biological samples (n = 3 mice / sample) differing from those employed for next generation sequence analyses. The U6 small nuclear RNA was employed as an endogenous control to normalize the expression levels of target miRNAs. * P < 0.05, ** P < 0.01, *** P < 0.001.