Rhomboid Enhancer Activity Defines a Subset of Drosophila Neural Precursors Required for Proper Feeding, Growth and Viability
Fig 4
Temporal control of toxin expression separates defects in development and viability.
(A-B) Temperature-sensitive Gal80 expression controls Gal4-dependent GFP reporter activity. Stage 17 embryos, z-projected dorsal views. (C-D) Quantification of pupae formed (C) and surviving to adulthood (D) for larvae subject to different toxin expression conditions. (E-G) Analysis of age at pupariation (E), length of pupation (F) and age at eclosion (G) for non-toxin-targeted and embryonically-targeted flies. Since developmental timing is temperature dependent, data are shown as experimentals (UAS-DTI positive) normalized to controls (UAS-DTI negative). *p<0.01 or **p<0.001. (H) Comparison of non-targeted and embryonically-targeted adults. Scale bar, 1 mm. (I) Quantification of wing area for non-targeted and embryonically-targeted flies. p>0.1 for inter-sex comparisons.