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Gene Expression Profile in the Liver of BALB/c Mice Infected with Fasciola hepatica

Fig 6

The microarray was validated with PCR reactions.

(A) PCR amplification from liver RNA samples for the selected genes at time t0 (before any treatment). (B) PCR amplification at t21 (21 days after oral infection of mice with F. hepatica metacercariae). Three up-regulated genes and three down-regulated genes were randomly chosen for PCR amplification. The corresponding PCR for each gene was performed using each biological sample obtained at t0 and t21. The results are representative of three individual experiments. MWM: molecular weight marker.

Fig 6

doi: https://doi.org/10.1371/journal.pone.0134910.g006