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Nuclear Receptor Expression and Function in Human Lung Cancer Pathogenesis

Fig 4

Characterization of tumorigenic HBEC clones.

(A) Histologic analysis of tumorigenic clones; C1 tumor poorly differentiated carcinoma with large cells suggestive of squamous cell carcinoma (H&E, x40) (left) and C5 tumor poorly differentiated carcinoma with features of adenocarcinoma (H&E, x40) (right). (B) In vitro characterization of HBEC tumorigenic clones C1 and C5. Immunoblot assays were performed using antibodies against K-ras, p53, pERK, total ERK, and beta-actin in tumorigenic HBEC clones. Using QPCR assay, the mRNA expression of PPARγ was measured in tumorigenic clones (bottom in B). (C) Growth response of tumorigenic HBEC clones to the combined treatment of PPARγ (3 μM troglitazone) and RXR (100 nM LG268) ligands. Data represent the mean ± SD (n = 3). Asterisks show a statistically significant point as evaluated by ANOVA. * P < 0.001 compared to HBEC-KT control.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0134842.g004