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Macrophage-Tumor Cell Fusions from Peripheral Blood of Melanoma Patients

Fig 2

Immunostaining of human melanoma cell lines SK-MEL-28 and SK-MEL-31.

Representative confocal images of human melanoma cell lines SK- MEL-28 and SK-MEL-31 are shown. Nuclei were stained with DAPI (Blue) shown in Panels [A, E, I, and M]. The same cells were also stained with various fluorescent markers specific for melanocyte, macrophage, or epithelial differentiation. Panel [B,C]: SK-MEL-28 stained with M2- polarization macrophage marker CD206 (Green) and melanocyte marker Melan-A. Panel [F,G]: SK-MEL-31 stained with M2-polarization macrophage marker CD206 (Green) and melanocyte marker Melan-A. Panel [J,K]: SK-MEL-31 stained with pro-carcinogenic cytokine MIF (Green) and pan-macrophage marker CD68 (Red, no signal on the majority of cells). Panel [N,O]: SK-MEL-31 stained with M2- polarization macrophage marker CD204 (Green) and epithelial cell adhesion molecule EpCAM (Red). Composite images are shown in Panels [D,H,L and P].

Fig 2

doi: https://doi.org/10.1371/journal.pone.0134320.g002