Ferritin Is Required in Multiple Tissues during Drosophila melanogaster Development
Fig 6
Ferritin genes interact genetically with the DMT1 homolog Mvl.
(A) The Mvl97f-LacZ line shows a spatially restricted expression pattern for Mvl, mainly in the head region, the brain, and a segmentally repeated pattern. (B) In a ferritin homozygous mutant background, Mvl97f-LacZ expression increases. Black arrows denote the head region, white asterisk the embryonic brain, red asterisk the ventral nerve cord, and red arrows mark the segmented expression pattern. Introduction of a Mvl97f allele into a ferritin mutant background resulted in the appearance of necrotic patches in the cuticle (C, D). (E) Quantification of the total area covered by LacZ staining in control and ferritin mutant backgrounds, (p<0.0001; T-test, asterisk). (F) Quantification of the number of embryos showing necrotic patches with one or two Mvl97f alleles; statistical difference using a Chi squared test with p<0.0001 is shown by an asterisk.