Functional Classification of Uncultured “Candidatus Caldiarchaeum subterraneum” Using the Maple System
Fig 1
Phylogenetic position of “Candidatus Caldiarchaeum subterraneum” based on concatenated common protein sequences.
A maximum-likelihood tree was constructed using MEGA [25]. The phylogenetic trees were collapsed at the family level except for Nanoarchaeum, “Ca. Korarchaeum,” and “Ca. Caldiarchaeum.” (A) Concatenated alignment of the sequences of 22 common proteins with no paralogs and domain splitting (hisS, pheS, valS, rpl11p, rpl14p, rpl1P, rpl22p, rpl2p, rpl3p, rpl5p, rpl6p, rplW, rps10p, rps11p, rps19p, rps2P, rps3p, rps4p, rps5p, rps7p, rps8p, and rps9p) among 142 archaea, including “Ca. A. autotrophicum” and two bacteria as an outgroup, as listed in S1 Table. (B) Concatenated alignment of the sequences of 12 common protein sequences without paralogs and domain splitting (eif2G, rfcL, eif6, rpl10e, rpl15p, rpl18p, rpl7ae, rplP0, rps19p, rps3p, rps5p, and rio1) among 137 archaea, including “Ca. C. subterraneum” and 13 unicellular eukaryotes as the outgroup (S1 Table). The numbers indicate the bootstrap support expressed as percentages. Bootstrap values of less than 50% were omitted.