Dichloroacetate, the Pyruvate Dehydrogenase Complex and the Modulation of mESC Pluripotency
Fig 2
(A)- Quantification for the alkaline phosphatase assay: colonies stained red were counted as (positive) pluripotent colonies whereas colonies without staining were counted as negative. A total of 10 independent experiments were analyzed for all experimental conditions resulting in a significant negative impact for CTR w/o LIF and DCA 5 mM. (B)- Quantification of pluripotency markers Oct4 and Nanog expression levels using high-resolution ELISA. All experimental conditions present a significant decrease when compared to the control for both pluripotency factors. (C)- qRT-PCR analysis for the Oct4 and Nanog mRNA gene levels results are represented as fold changes normalized to the Control after normalization for endogenous βeta-actin. These results mirror the protein levels regarding CTR w/o LIF and DCA 5 mM. Four independent experiments were performed. All the results are expressed as means and error bars represents SEM. * and # < 0.05; ** and ## p< 0.01; *** and ### p< 0.001 relative to the respective controls.