Nano-Drugs Based on Nano Sterically Stabilized Liposomes for the Treatment of Inflammatory Neurodegenerative Diseases
Fig 7
Comparison of the therapeutic efficacy of NSSL-MPS and free MPS in the adoptive transfer EAE mice model.
(A) SJL mice were treated by IV injections on days 8, 10, 12 post-T cell transfer with saline (control) (▲), free MPS (50mg/kg) (■) or NSSL-MPS (50mg/kg) (◆). (B) Treatment with NSSL-MPS reduced inflammation and demyelination in brains and spinal cords of mice with AT-EAE compared with free MPS treated mice and control mice. Brains and spinal cords were obtained on day 13 post T- cell transfer. Black arrows indicate infiltrating inflammatory cells; white arrow indicates demyelination. Representative H&E-stained brains (A,B,D,E,G) and spinal cord (C,F,H) sections from control (A-C), as well as NSSL-MPS (D-F) and free MPS treated EAE mice (G-H) show extensive inflammation involving perivascular infiltrates of mononuclear leukocytes (arrows) within the cerebral parenchyma and spinal cords of CTRL mice and free MPS treated mice (arrows). In the NSSL-MPS treated group, much fewer infiltrating cells were observed. LFB staining demonstrates extensive demyelination in the control spinal cord (J) and a decrease in myelin density in the free MPS treated group (I,L) around blood vessels compared with NSSL-MPS treated mice (K), demonstrating densely organized myelin sheaths. Original magnification of x 40.