Hepatic Progenitor Cells Contribute to the Progression of 2-Acetylaminofluorene/Carbon Tetrachloride-Induced Cirrhosis via the Non-Canonical Wnt Pathway
Fig 1
2-AAF promoted the inflammatory reaction.
(a) H&E staining (×200). (b) A higher magnification of the squared area (×800). (c) CD68 immunostaining (×200) during cirrhosis progression. (d) A higher magnification of the squared area (×800). (e) Immunoblotting for CD68 and TNF-α. (f) The gray-level score indicates the immunoblotting histogram for CD68 and TNF-α. (g) Relative expression levels of CD68, CD163 and TNF-α were measured by qRT-PCR. *p < 0.05, **p < 0.01. Negative, Negative control; N, normal group; 8wM, the 8th week CCl4 group; 12wM, the 12th week CCl4 group.; 2-AAF(L), the 2-AAF(L) group; 2-AAF(H), the 2-AAF(H) group.