Isolation and Characterization of a Novel Pathogenesis-Related Protein Gene (GmPRP) with Induced Expression in Soybean (Glycine max) during Infection with Phytophthora sojae
Fig 3
Relative expression of GmPRP mRNA in leaves of soybean plants after abiotic or biotic stress.
(A) Infection study. The leaves of ‘Suinong 10’ soybean seedlings inoculated with zoospores of P. sojae race 1 were also harvested at 6, 12, 24, 36, 48, and 72 h. (B) Tissue-specific expression study. GmPRP expression in the organs of sterile seedlings. (C) Hormone study. The leaves were collected from soybean plants at 1, 3, 6, 9, 12, and 24 h after treatment with ABA (50 mM) (a), SA (0.5 mM) (b), ET (5%(v/v)) (c), or JA (100 mM) (d). The experiments were performed at least three times for each treatment. Data shown are representative results. Statistical analyses were performed using Student’s t-test (*P < 0.05, **P < 0.01). Bars indicate the standard error of the mean (SE).