MiR-133a Is Functionally Involved in Doxorubicin-Resistance in Breast Cancer Cells MCF-7 via Its Regulation of the Expression of Uncoupling Protein 2
Fig 3
Knockdown of UCP-2 in MCF-7/DOX restores its Doxorubicin sensitivity.
(A) The mRNA and protein expression levels of UCP-2in MCF-7/Dox cells infected with lentivirus expressing eithershUCP-2 or scramble shRNA for 72 h were measured by real-time PCR (Left) and western blot analysis (Right) respectively. (B) Cell growth curve plotted with total cell amount of MCF-7/Dox cells infected with lentivirus expressingscramble shRNA and shUCP-2by cell counting in connective 8 days treated with 0.4 nM Doxorubicin (Left).Cells were seeded in amount according to their doubling time to ensure comparable amount in the starting day of treatment. Right, cell viability under the treatment of Doxorubicin was shown as folds change of cell viability normalized to that of the cells treated with saline. Each bar represents the mean ± SEM. The results shown were repeated in three independent experiments. *, P < 0.05; **, P<0.01 and***, P < 0.001 significantly different from the respective control group.