p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA
Fig 1
Identification of p53 regulated snoRNAs.
(A) Wild type p53-inducible H1299 cells were treated with 2.5μg/ml of Ponasterone A (PonA) for 24 hours, and compared with non-induced cells. WE-68 cells were treated with 10nM Nutlin-3a for 16 hours, compared with non-induced cells. Western blots for p53 (and β-actin as a loading control) are shown. (B) Based on microarray profiling, a dendrogram generated by cluster analysis shows the separation of p53 uninduced cells from induced cells, and separation of representative genes activated, repressed or not significantly changed by p53. (C) SNHG1 expression levels were determined by RT-PCR after p53 induction in H1299 and WE-68 cells. (D) ChIP assay, using the anti-p53 antibody DO-1, was performed to determine relative p53 occupancy in p53-induced H1299 cells (+PonA). RT-PCR results show relative p53 occupation at upstream of the SNHG1 promoter, and p53 null H1299 (-PonA) was used as a negative control. ** p<0.01 versus controls for all experiments.